1st NYRA-EAA-Andronet School ‘Omics of male germ cells: Transcriptomic analyses’ Program

DAY 1 – FEB 24, 2025

12:45 – 13:00 | Registration

13:00 – 13:10 | Opening and welcome

13:10 – 14:30 | The complexity of the testis transcriptome and approaches to its study

14:30 – 15:00 | Coffee break

15:00 – 17:00 | Leveling our programming in R and Python: essential concepts

DAY 2 – FEB 25, 2025

09:00 – 10:30 | Bulk RNA-sequencing: principles and data processing

10:30 – 11:00 | Coffee break

11:00 – 12:30 | Bulk RNA-sequencing: differential analyses

12:30 – 13:30 | Lunch break

13:30 – 15:00 | Bulk RNA-sequencing: downstream analyses (in groups) Part 1

15:00 – 15:30 | Coffee break

15:30 – 17:00 | Bulk RNA-sequencing: downstream analyses (in groups) Part 2

DAY 3 – FEB 26, 2025

09:00 – 10:30 | Single-cell RNA-sequencing: fundaments of the technique, sample preparation

10:30 – 11:00 | Coffee break

11:00 – 12:30 | Single-cell RNA-sequencing: data processing

12:30 – 13:30 | Lunch break

13:30 – 15:00 | Single-cell RNA-sequencing: integration and quality controls (Part 1)

15:00 – 15:30 | Coffee break

15:30 – 17:00 | Single-cell RNA-sequencing: integration and quality controls (Part 2)

DAY 4 – FEB 27, 2025

09:00 – 10:30 | Single-cell RNA-sequencing: data visualization

10:30 – 11:00 | Coffee break

11:00 – 12:30 | Single-cell RNA-sequencing: downstream analysis (Part 1)

12:30 – 13:30 | Lunch break

13:30 – 15:00 | Single-cell RNA-sequencing: downstream analysis (Part 2)

15:00 – 15:30 | Coffee break

15:30 – 17:00 | New technologies and perspectives on transcriptional studies

19.00 – 20:45 | Networking dinner

DAY 5 – FEB 28, 2025

09:00 – 10:30 | Wrap-up presentations from the students

10:30 – 11:00 | Coffee break

11:00 – 12:30 | Discussions and Q&A

12:30 – 12:40 | Closing and group picture